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The PrimerQuest Tool offers 4 design options that are based on algorithms specific for common experimental setups (Figure 1). ladangtoto2 wap By default, your results return the 5 best primer or assay designs. ladangtoto2 wap PCR (2 primers) qPCR (2 primers + probe; for use in 5′ nuclease assays) qPCR (2 primers; for use with intercalating dyes)